Rabbit Anti-T7 RNA Polymerase Antibody

Customer Review

There are currently no Customer reviews or questions for MOB-0296MC. Click the button above to contact us or submit your feedback about this product.

Rachel Adams
10.Feb,24

Great for Polymerase Detection

The Anti-T7 RNA Polymerase Antibody has been fantastic for our polymerase detection assays. Its specificity and sensitivity are outstanding, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

John Lee
15.Jun,23

Reliable and Effective

We incorporated this antibody into our Western blot assays, and it has been a great addition. The specificity for T7 RNA polymerase is precise, consistently detecting the target protein with high clarity. The reliable performance has made it an essential tool in our research.

Megan Walker
01.Nov,22

Consistent High-Quality Results

This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for T7 RNA polymerase have been particularly beneficial.

User 185*****74
04.Dec,24

Fluorescent Immunochemistry for MOB-0296MC

The antibody worked well in our assays.

Q&As

1. Is the anti-T7 RNA polymerase antibody suitable for use in Western blotting?

   A: Yes, the anti-T7 RNA polymerase antibody (MOB-0296MC) is suitable for use in Western blotting. It provides specific binding to T7 RNA polymerase, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-T7 RNA polymerase antibody?

   A: The recommended storage condition for the anti-T7 RNA polymerase antibody (MOB-0296MC) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-T7 RNA polymerase antibody be used in immunoprecipitation assays?

   A: Yes, the anti-T7 RNA polymerase antibody (MOB-0296MC) can be used in immunoprecipitation assays. It provides specific binding to T7 RNA polymerase, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-T7 RNA polymerase antibody effective in ELISA applications?

   A: Yes, the anti-T7 RNA polymerase antibody (MOB-0296MC) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of T7 RNA polymerase.

5. What is the optimal dilution for using the anti-T7 RNA polymerase antibody in Western blotting?

   A: The optimal dilution for using the anti-T7 RNA polymerase antibody (MOB-0296MC) in Western blotting is typically 1:500 to 1:2000. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Dukhovny, Anna, Amir Shlomai, and Ella H. Sklan. "The antiviral protein Viperin suppresses T7 promoter dependent RNA synthesis-possible implications for its antiviral activity." Scientific Reports 8.1 (2018): 8100. https://doi.org/10.1038/s41598-018-26516-z
   This study explores the antiviral activity of viperin, an interferon-inducible protein, focusing on its ability to inhibit RNA synthesis mediated by bacteriophage T7 polymerase in mammalian cells. The researchers demonstrated that viperin significantly reduces cytoplasmic RNA levels by specifically inhibiting T7 polymerase-dependent RNA synthesis without affecting RNA polymerase II-dependent transcription. This inhibition is shown to be specific to viperin, as other interferon-stimulated genes did not exhibit similar effects. The study also investigated the structural domains of viperin necessary for its inhibitory activity, finding that mutations in these domains abolished its function. This research provides insights into the broad-spectrum antiviral mechanisms of viperin and its potential role in inhibiting viral RNA synthesis across different species.
   Creative Biolabs provided the anti-T7 polymerase antibody (Cat# MOB-0296MC) used in the Western blot analysis. This antibody was crucial for detecting T7 polymerase protein levels in various experimental setups, allowing the researchers to confirm that viperin does not affect the stability of T7 polymerase but specifically inhibits its RNA synthesis activity. By enabling accurate detection and analysis of T7 polymerase, Creative Biolabs' antibody supported the study's investigation into the mechanisms underlying viperin's antiviral functions.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MOB-0296MC, RRID: AB_3111474)

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

See other products for "T7 RNA Polymerase"

Select a product category from the dropdown menu below to view related products.